The invention belongs to the technical field of analysis, and particularly relates to a method for measuring the content of styrene in a sample. The method comprises the following steps of: weighing acertain mass of sample to be digested, adding concentrated nitric acid for microwave digestion, and digesting and converting the styrene in the sample into a digestion solution containing multiple nitrobenzoic acids; preparing the digestion solution into a sample solution to be measured, and performing high performance liquid chromatography test to obtain a liquid chromatogram of the sample solution to be measured; and calculating the mass concentrations of four nitrobenzoic acids in the sample solution to be measured by combining the peak area corresponding to each nitrobenzoic acid in the liquid chromatogram of the sample solution to be measured with a standard curve of the four nitrobenzoic acids, and calculating according to a formula (I). The pretreatment steps are simplified, so that the pretreatment is simpler and quicker; the interference of similar substances is eliminated, the experimental repeatability and reproducibility are good, and the accuracy is high; and the problemthat the measurement result of high-content styrene is inaccurate is solved.