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CN1271621A 用于从血浆中去除致病抗体及其复合物的蛋白免疫吸附介质的合成方法 失效 - 权利终止

用于从血浆中去除致病抗体及其复合物的蛋白免疫吸附介质的合成方法
摘要：
一种用于从血浆中去除致病抗体及其复合物的蛋白免疫吸附介质的合成方法,合成过程依下述步骤进行:(1)用环氧氯丙烷活化Sepharose CL－4B琼脂糖凝胶,反应温度40～80℃,时间1～4小时,环氧氯丙烷充分过量;(2)用环氧Sepharose CL－4B琼脂糖凝胶与ProteinA直接反应合成免疫吸附介质,反应温度为24～26℃,反应时间16～20小时,ProteinA充分过量,pH值控制在9～10范围内;用甘氨酸乙酯盐酸盐或乙醇氨封闭未反应活性基团;放入保存液中。本发明可以取代溴化氰活化工艺,无毒、无害,且偶联Protein A效果好。
摘要（英）：

A process for synthesizing a protein immunoadsorbent medium to remove pathogenic antibody and its compounds from plasm includes such steps as activating Sepharose CL-4B with sufficiently excess epoxychloropropane at 40-80 deg.C for 104 hr; direct reaction of epoxy Sepharose CL-4B on sufficiently excess Protein A at 24-26 deg.C for 16-20 hr while controlling the pH value at 9-10; sealing the non-reactive active radicals with ethylglycinate hydrochloride or alcohol, and putting in retained liquor. Its advantages are no poison and harm, and high coupling effect of Protein A.

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