A process for preparing the biologic silica gel and containing dehydrogenase includes reacting of its precursor on water under the step catalysis of catalyst to generate the sol of silicon oxide, mixing it with the solution of zymogen in buffering liquid to generate gel, and laying aside for ageing. The said precursor is methyl n-silicate, ethyl n-silicate, or propyl n-silicate. The said catalystis hydrochloric acid, sulfuric acid, nitric acid, acetic acid, sodium hydroxide, ammonia water, phosphate, or acetate. Its advantages are short time, big average pore diameter, and high maintained activity of enzyme.