利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN1448504A 工程菌LZ－17的构建和应用 有权

工程菌LZ－17的构建和应用
摘要：
本发明涉及工程菌LZ－17的构建和应用，属生物工程或基因工程技术领域。用分子克隆技术，将青海弧菌的发光基因导入鼠伤寒沙门氏菌TA97，构建成工程菌LZ－17。该工程菌能产生细菌荧光酶，加入葵醛后能发出波长为440～640nm的可见光；具备鼠伤寒沙门氏菌TA97所具有的与Ames试验有关的性状。该工程菌作为受试生物应用在Ames试验中，可采用发光检测确定回变细菌的数量。发光Ames试验具有发光检测可自动化操作，省时、省力和细菌培养时间短等优点。该工程菌特别适于用来检测被检样品的致突变性。
摘要（英）：

The present invention relates to the construction and application of engineering bacterium LZ-17, and belongs to the field of bioengineering and gene engineering technology. By means of molecular cloning technology, the luminous gene of Qinghai vibrio is introduced into Salmonella typhimurium TA97 to constitute engineering bacterium LZ-17. The engineering bacterium can produce bacterioleuciferinase, can glow with visible light of 440-640 nm wavelength after decanol is added, and has the Ames test relevant characters the same as Salmonella typhimurium TA97. The engineering bacterium is applied in Ames test as testee life form for determining the number of back varying bacterium. The engineering bacterium is especially suitable for detection of mutagenicity of the testee sample.

公开/授权文献
0/0