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CN1515666A 用于防治青枯病的假单胞菌的筛选方法 无效 - 驳回 复审申请

用于防治青枯病的假单胞菌的筛选方法
摘要：
本发明属于细菌的筛选方法。其步骤为：①在青枯病发生的田块中采集发病株和健康株的根、茎和根围土；②从中分离菌株；③通过分离菌株间的平皿相互拮抗试验和分离菌株的致病性试验区分出拮抗菌株和病原菌株；④以拮抗率、反拮抗率分析拮抗菌、病原菌和其他类之间相互关系，选出属假单胞杆菌或芽孢杆菌的拮抗菌株；⑤以田间防效不小于60％为基准对选出的拮抗菌株进行挑选，所得到的菌株即为有效菌株。以通过本发明的方法筛选出的防治青枯病的假单胞菌为主要成分的微生物制剂，是一种高效、无毒、安全、无残留的新型微生物农药。对因青枯假单胞菌引起的烟草、番茄、花生、茄子、辣椒等植物的青枯病有很强的抑制作用，田间防效达60～80％左右。
摘要（英）：

The method for screening pseudomonas for controlling bacterial wilt includes the following steps: (a). collecting root, stem and root medium soil of disease plant and health plant in the field with bacterial wilt; (2). separating strains; (3). utilizing Petri dish nutual antagonistic test between separated strains and pathogenicity test of separated strains to identify antagonistic strain and pathogenic strain; (4) using antagonistic rate and anti-antagonistic rate to analyze mutual retationship between antagonistic strain, pathogenic strain and others and select the antagonistic strains belonging to pseudomonas or bacillus; and (5). using the field controlling effect whis is whis is not less than 60% as reference selecting the antagonistic strain so as to obtain the effective strain.

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