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发明公开
CN1526733A 抗人多囊蛋白－1 N端多克隆抗体PAPC1 无效 - 撤回

抗人多囊蛋白－1 N端多克隆抗体PAPC1
摘要：
本发明涉及医药分子生物学、免疫学检测技术领域，是一种抗人多囊蛋白－1N端多克隆抗体PAPC1，可用于制备研究常染色体显性遗传性多囊肾病发病机制的试剂。本发明根据人类多囊肾病1基因(PKD1)的cDNA序列和其编码产物——多囊蛋白－1(PC－1)的分子结构，采用分子生物学技术克隆到编码PC－1的cDNA，并构建其原核表达载体，在大肠杆菌中诱导表达出PC－1N端的融合蛋白(PC－1的flank－LLR－flank区和部分WSC区)，以此为抗原免疫动物制备抗血清，并对其进行纯化得到多克隆抗体PAPC1。经针对多囊蛋白－1N端的综合性能指标测试，该抗体在免疫组织化学、酶联免疫吸附、免疫印记和免疫沉淀实验中性能稳定，敏感性高，特异性强，故可用于制备研究常染色体显性遗传性多囊肾病发病机制的试剂。
摘要（英）：

The present invention relates to medicine molecular biology and immulogical detection technology, and is one kind of 1N-end polyclonal antibody PAPC1 of anti-human polycystic protein-1. According to cDNA sequence of human polycystic kidney disease-1 gene and the molecular structure of its encoded product, the gene is cloned to cDNA encoding PC-1 and its prokaryotic expression carrier is constituted by means of molecular biology technology. N-end fusion protein of PC-1 is induced in colibacillus and used as antigen for immunizing animal to prepare antiserum and is purified to obtain polyclonal antibody PAPC1. Comprehensive performance test shows that the antibody has stable performance, high sensitivity and powerful specificity in immunohistochemistry, enzyme-linked immunosorbent assay, immunoblotting and immunoprecipiation experiments, so that it may be used in preparing reagent for polycystic kidney disease mechanism research.

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