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发明公开
CN1528469A 具备生物活性同种骨组织的制备及保存方法 无效 - 撤回

具备生物活性同种骨组织的制备及保存方法
摘要：
本发明涉及一种具备生物活性同种骨组织的制备及保存方法，其步骤为：1.生物活性骨组织的获取：在无菌条件下获取同种骨关节组织；2.生物活性骨组织的修整：剔除软组织及骨膜，清除骨髓组织，保留部分关节囊、韧带和肌肉的附着点；3.生物活性骨组织的脱脂：氯仿甲醇浸泡，生理盐水冲洗后包装；4.梯度降温：将预处理后的骨组织放置程序降温仪中，以0.5～2℃/min的速率梯度降温至－40℃～－60℃，历时20－80min；5.保存：将降温后的骨组织投入－60～80℃冰箱中保存。本发明能够保持骨的超微结构的完整性、骨诱导活性、生物力学强度，同时维持一定数量的成活的软骨细胞，能够增加移植后与宿主的愈合能力，减少并发症的发生率，改善移植后的关节功能。
摘要（英）：

The invention relates to a method to prepare and preserve homogeneous bioactive bone tissue, its steps: 1, obtaining: on the aseptic condition, obtaining homogeneous bone tissue; 2, finishing: eliminating soft tissue and periosteum, eliminating bone marrow tissue, and reserving partial joint bursa, ligament and adhesive point of muscle; 3, defatting: dipping in chloroformic methanol, washing with physiological saline and then packaging; 4, gradient temperature reducing: placing the preprocessed bone tissue in program temperature reducer to make gradient temperature reduction up to -40 deg.C--60 deg.C for 20-80 min.; 5, preserving: putting the bone tissue in the -60 deg.C - 80 deg. C refrigerator. It can keep the integrality of bone ultramicro structure, bone inducing activity, bioforce intensity and maintain a certain quantity of survival soft bone cells.

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