The card type biological identification device includes one biological sensor, one operating and processing module, one radio transceiver and one rechargeable battery. The biological sensor is used to read the biological data of the card holder. The operating and processing module has stored random code generating program, personal data and authorized biological data, accepts and processes biological data to be processed, and generates and outputs codes to be identified after judging that the biological data accords with the authorized biological data. The radio transceiver outputs the personal data to a host computer system, so that the host computer produces one authorizing code based on the stored corresponding personal data random code and compares the authorized code with the code to be identified to judge whether the holder is authorized holder.