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发明公开
CN1611944A 甘蔗花叶病毒云南分离物TAS－ELISA检测试剂盒及其制备方法 失效 - 权利终止

甘蔗花叶病毒云南分离物TAS－ELISA检测试剂盒及其制备方法
摘要：
本发明公开了一种甘蔗花叶病毒云南分离物TAS－ELISA检测试剂盒，包括盒体、设在盒体内的酶标板和设在盒体内的用液，盒体内的用液包括阳性对照、阴性对照、酶标抗体、缓冲液和底物，盒体内的用液还包括甘蔗花叶病毒云南分离物多克隆抗体、甘蔗花叶病毒云南分离物单克隆抗体，所述的甘蔗花叶病毒多克隆抗体和所述的甘蔗花叶病毒单克隆抗体由35～45mg/ml病毒浓度的甘蔗花叶病毒提取液作为免疫抗原，分别采用免疫注射法、单克隆抗体法制备而成；本发明所述的检测试剂盒检测灵敏度高，检测灵敏度可达到0.005～0.01ng/ml，比间接ELISA和DAS－ELISA检测灵敏度提高100～1000倍。
摘要（英）：

This invention discloses a kin of sugar-cane mosaic virus yunnan outlier three antibody sandwich enzyme immune adsorbing rule detection kit. It includes case, enzyme standard board and liquid set in the case. The liquid comprises positive and negative contrast, enzyme standard antibody, buffer solution and substrate. It also includes multiple and single antibodies of sugar-cane mosaic virus yunnan outlier. The single and multiple antibodies take sugar-cane mosaic virus extracting solution with 35-45 mg/ml virus concentration as immune antigen. They are prepared by immune injection method and single clone antibody method. The detection sensitivity of the detection kit in this invention can reach 0.0051-0.01ng/ml, and it is 100-1000 times than indirect ELISA and DAS-ELISA detection sensitivity.

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