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发明公开
CN1621839A 氯菊酯多克隆抗体酶联免疫检测方法 无效 - 撤回

氯菊酯多克隆抗体酶联免疫检测方法
摘要：
本发明公开了一种氯菊酯多克隆抗体酶联免疫检测方法，利用合成的氯菊酯免疫抗原免疫健康白兔得到多克隆抗体，筛选后以氯菊酯为标准品，以氯菊酯半抗原与OVA的偶联物作为包被抗原，建立土壤和食品中氯菊酯农药的间接竞争酶联免疫法。本发明建立了土壤和食品中氯菊酯农药的间接ELISA法，为氯菊酯在土壤和食品中的残留检测提供了快速高效的检测手段，由于采用的是多克隆抗体，费用较低并且稳定性和重复性较好。灵敏度为0.1ppb，线性范围为10～800ppb。免疫反应的高特异性和高亲和性使ELISA具有极高的选择性和灵敏性，样本前处理过程简单。
摘要（英）：

The present invention discloses one kind of permethrine polyclonal antibody enzyme-linked immunoassay method. Synthetic permethrine immune antigen is utilized in immunizing health rabbit to obtain polyclonal antibody, and after screening, the indirect competitive enzyme-linked immunoassay method for detecting residual permethrine in soil and food is established with permethrine as standard article and permethrine semi-antigen and OVA conjugate as coating antigen. The method is fast and efficient, and has relatively low cost, high stability and high repeatability, sensitivity of 0.1 ppb, linear range of 10-800 ppb, high selectivity and simple sample pre-treatment.

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