The invention relates to a restructuring poxvirus vector which can express two or more homologous or exogenous sequences, wherein, the exogenous sequences originate from the different varieties of microorganisms, with fifty percent or more homology inbetween. The invention further relates to a method for preparing the restructuring poxvirus and the application of the restructuring poxvirus as medicine or vaccine. Furthermore, the invention also provides a method for influencing preference for inducing the immune response for living animals including human.