Separation of rebescensine standard check products relates to production from blushed rabdosia. The process is carried out by reflux extracting for broken blushed rabdosia, concentrating to obtain heavy cream extractive, de-fatting and chromatographing on silica-gel column, desorbing, de-pressuring and concentrating, laying aside and crystallizing to obtain blushed rabdosia crude crystal with content >90%, dissolving from methanol, chromatographing on silica-gel column, washing pre-impurity from mineral ether, gradient desorbing from mixed solvent of mineral ether and acetone, merging desorbing liquid containing rebescensine, concentrating and crystallizing, de-pressuring, pumping and filtering, separating crystal from mother liquid, concentrating and crystallizing mother liquid, repeating for many times until no crystal precipitating, merging crystal, and re-crystallizing and refining from mixed solvent of acetone and methanol. It achieves low cost and less solvent.