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CN1769295B 使用纯化的抗原特异性抗体快速检测肺炎链球菌的体外方法和材料 失效 - 权利终止 转让

使用纯化的抗原特异性抗体快速检测肺炎链球菌的体外方法和材料
摘要：
公开了一种肺炎链球菌的纯化的细胞壁 C- 多糖抗原，其含有不多于 10%重量的蛋白质，如下获得：（a）处理肺炎链球菌培养物，杀死细菌细胞；（b）在约 7.0-7.4 的弱碱性 pH条件下，分离作为湿细胞沉淀的被杀死的细菌细胞；（c）将沉淀与足量的 0.1N NaOH 一起保温至少 45 分钟，所述 0.1NNaOH 将其 pH 提高到超过 12.0 ，溶液中混合；（d）用强酸将溶液调节到 pH3以下，诱使其分层；（e）分离上清液，并用强蛋白酶处理，除去蛋白质；（f）分离出肺炎链球菌的纯化的细胞壁 C- 多糖抗原，它含有不多于10% 重量的蛋白质。还公开了在动物体内产生的针对肺炎链球菌或其细胞壁 C- 多糖抗原的多价抗体。
摘要（英）：

The present invention discloses a cell wall C-polysaccharide antigen of streptococcus pneumoniae which contains not more than 10% by weight of protein which has been obtained by a) subjecting a culture of S. pneumoniae to a treatment which kills the bacterial cells, b) separating the killed bacteria cells as a cell pellet of weakly alkaline pH in the order of from about 7.0 to 7.4, c) subjecting the pellet to incubation with a sufficient quantity of 0.1 N NaOH to elevate its pH to a value in excess of 12.0 for a period of at least 45 minutes, d)adjusting the solution below pH3 with strong acid and delaminating the same, e) separating the upper clear solution and deproteinizing with strong prolease process, f) separating out a purified cell wall C-polysaccharide antigen containing not morethan about 10% by weight of protein. The invention also discloses a polyvalent antibody generated in animal body aiming at streptococcus pneumoniae or its cell wall C-polysaccharide antigen.

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