The invention discloses a heat preservation tyrosine protein phosphatase generated from popillia japonica green muscardine fungus strain CQMa102 and its separation purifying method. It adopts special induced customs agency to induced culture the popillia japonica green muscardine fungus to form the enzyme and obtained by separation purifying. The enzyme protein is glycoprotein, which can characteristic hydrolyze the phosphate radical group of tyrosine; the N-ethyl maleimide, molybdate, vanadate and divalent zinc ion can characteristic depress the activity of the enzyme; the fluoride has non appreciable effect to the activity of the enzyme which can be used in biochemical reagent.