This invention relates to a knit fabric, in particular to a single floss knit fabric without bias weft. It comprises the following steps: not twisting silk yarns, twisting cotton yarns, shaping twisted cotton yarn with steam, forming a strand of inlet yarns with 2-4 silk yarns without twisted and 1 left-twisted cotton yarn, forming another strand of inlet yarns with the same numbers of silk yarns and 1 left-twisted cotton yarn, knitting them on the single circular knitting machine or stillage truck. The floss knit fabric has the character of good elasticity, good attribute, and no bias weft ratio.