This is the method of preparation rapeseed peptides by the direct enzymatic hydrolysis of rapeseed meal. This invention can resolve the problem that the existing method of preparation rapeseed peptides with rapeseed meal need extract rapeseed protein from rapeseed meal, and then perform enzymatic hydrolysis, and which is too long and too expensive. The features lays that: (1) comminution the rapeseed meal that through pre-pressing, oil Extraction or dehulling, low temperature pressing, oil extraction; (2) immerging and washing the comminuted rapeseed meal with acid water, then get the wet rapeseed meal that removed the anti-nutritional factors such as phytate and glucosinolate after isolating the wasted washing water; (3) adding water and protease to enzymatic hydrolyse the wet rapeseed meal, then inactivation by heating and make centrifugal isolation to remove the residue, the hydrolysed liquid is the crude product of rapeseed peptide. This invention can prepare rapeseed peptides by the direct enzymatic hydrolysis of rapeseed meal. It can shorten the process and lower cost.