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发明授权
CN1891836B 定量测量固定的石蜡包埋的组织样品中靶基因表达的方法 失效 - 权利终止

定量测量固定的石蜡包埋的组织样品中靶基因表达的方法
摘要：
本发明涉及用于定量测量固定的石蜡包埋的组织样品中靶基因的基因表达的方法。其包括：(a)将所述样品脱蜡，以获得脱蜡样品；(b)如下从所述脱蜡样品中分离mRNA：首先将所述组织样品在包含有效浓度的离液剂的离液溶液中加热至约50℃～约100℃的温度并持续约5分钟～约120分钟的时间，并从所述离液溶液中回收所述mRNA，以产生分离的mRNA；和(c)用能够扩增所述靶基因mRNA的区域的一对寡核苷酸引物对所述分离的mRNA进行扩增，以获得扩增样品；(d)测定来自所述分离的mRNA的靶基因mRNA的量与内控基因mRNA的量的比值。
摘要（英）：

The invention relates to a method for quantitative measurement of gene expression of target genes based on paraffin-embedded tissue specimens. The method comprises: (a) deparaffinizing the sample andobtaining a deparaffinized sample; (b) separating mRNA from the deparaffinized sample by heating the sample for about 5 to about 120 minutes at a temperature of about 50 to about 100 DEG C in the isolated solution containing isolated liquid formulation with effective concentration, and recovering the mRNA from an isolated solution to generate an isolated mRNA; and (c) using a pair of oligonucleotide primers capable of magnifying the target genes mRNA region to magnify the isolated mRNA, and obtaining an magnified product; and (d) testing quantitative ratio of the target genes mRNA from the isolated mRNA to internally piloting genes.

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