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发明公开
CN1953992A 源自胎盘和脑垂体同种型的嵌合体人生长激素和获得所述嵌合体的方法 无效 - 撤回

源自胎盘和脑垂体同种型的嵌合体人生长激素和获得所述嵌合体的方法
摘要：
本发明涉及具有编码人生长激素的SEQ ID NO：3的新的hGH－NV cDNA嵌合体。本发明还涉及用于由人胎盘和垂体RNA的cDNA同种型获得新的hGH－NV cDNA嵌合体的方法：通过使用基因特异引物由反转录扩增该cDNA同种型，克隆该已扩增的cDNA同种型，在特异限制性内切核酸酶位点消化该已克隆的cDNA，随后用hGH－N片段替换该已消化的cDNA同种型hGH－V片段特异区域以获得hGH－NV cDNA嵌合体。此外，该新的hGH－NV cDNA嵌合体用作模板以获得含有具有SEQ ID NO：5的片段的表达结构，以产生成熟人生长激素。
摘要（英）：

The present invention relates to a novel hGH-NV cDNA chimera having SEQ ID NO: 3 encoding human growth hormone. The invention also relates to a process for obtaining novel hGH-NV cDNA chimera from the cDNA isoforms of human placenta and pituitary RNA, by amplifying the cDNA isoforms by reverse transcription using gene specific primers, cloning the amplified cDNA isoforms, digesting the cloned cDNA isoforms at specific restriction endonuclease sites followed by replacing the digested cDNA isoform hGH-V fragment specific region by a hGH-N fragment to obtain hGH-NV cDNA chimera. Further the novel hGH-NV cDNA chimera is used as a template to obtain an expressible construct containing the fragment having SEQ ID NO: 5 to produce mature human growth hormone.

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