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发明授权
CN1986817B 植物油体表达载体及利用植物油体表达人酸性成纤维细胞生长因子的方法 失效 - 权利终止

植物油体表达载体及利用植物油体表达人酸性成纤维细胞生长因子的方法
摘要：
本发明采用体外基因重组方法，将人酸性成纤维细胞生长因子(Human acidic fibroblast growth factors haFGF)与油体蛋白基因融合合成，构建成一种植物油体表达载体，建立一种油体蛋白遗传转化表达体系，用根瘤农杆菌转化了拟南芥、红花、大豆、黑豆、油菜，并获得了转基因植株，转基因红花、油菜的分子生物学检测证明外源基因的整合和表达，经活性检测，获得的重组蛋白具有生物活性，其生物活性与人aFGF标准品活性相当。本发明还提供一种方法在植物种子中表达酸性成纤维细胞生长因子，这种方法可以提供一种经济的方法生产酸性成纤维细胞生长因子，可开发出一种医类新药。
摘要（英）：

The present invention is gene recombination process, in which human acidic fibroblast growth factors (haFGF) and oil-body protein gene are fused and synthesized to constitute one plant oil-body expressing vector and establish oil-body protein genetically transforming and expressing system. Root nodule agrobacterium is used to transform Arabidopsis thalianuum, safflower, soybean, black bean and rape to obtain transgenic plant. The molecular biology detection on transgenic safflower and rape proves the integration and expression of exogenous gene, and the activity detection proves the bioactivity corresponding to the standard haFGF activity of obtained recombinant protein. The present invention also provides the method of expressing aFGF in plant seed, and the method may be used in producing aFGF at low cost and developing new medicine.

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