The utility model relates to an electron beam irradiation sterilizing device, which comprises an electron beam irradiation accelerator and the control system, a frame trolley, a chamber arranged on the frame trolley, a ventilation device arranged in the chamber and a power system, the electron beam irradiation accelerator is positioned in the chamber. The utility model has the advantages of small in size, mobility and agility, realizing the fast transfer in a short time, because of the electron beam irradiation accelerator and the control system, the ventilation system, and the power system arranged inside the frame trolley chamber, has no special requirement for the operation environment, and no need extra construction. The utility model can do the electron beam irradiation on the variable files and post mails, realizes to sterilize fast and thoroughly, then achieves the safety without damage and residual on the irradiated objects; the utility model has a high automation integration, easy operation and safety, and is applicable to the domestic and overseas key department.