The utility model relates to a disposable mid-portion urine germiculture collector, which comprises an inner box (1) used for containing urine. The disposable mid-portion urine germiculture collector is characterized in that the inner box (1) is arranged in an outer box (2); a specimen outlet (3) and a specimen outlet cap (4) are arranged above the outer box. The disposable mid-portion urine germicuture collector is carried and used conveniently, which is not subject to pollution and can prevent cross infection.