The utility model relates to an acoustic positioning responder for a cymoscope used in secondary positioning earthquake exploration. The technical scheme is as follows: an acoustic transducer, a circuit cabin, a battery cabin and a dismountable seal choke plug are arranged in a shell; after the acoustoelectric conversion of the transducer, front filter, AGC amplifying, narrow belt filter, secondary amplifying and wave detection and shaping are carried out on a received sonar signal; the received sonar signal enters a SCM; after decoding judging is detected in the chip of the SCM and the judging is correct, the SCM transmits a self identifying code through a digital IO port, enters the transducer through a drive circuit and a push-pull amplifier circuit; then an emission sonar signal is converted through acoustoelectric conversion. The acoustic positioning responder has the beneficial effects of: enlarging the grade of the emission source and the receiving sensitivity of a system through the combination of hardware and software and improving the anti-interference capacity of the circuit by adopting a floating threshold and a software detection technology, thereby meeting the demands of high yield and high positioning efficiency for secondary positioning.