An oil well separate-layer proration regulator comprises an upper joint 1, a central pipe 2, an outer pipe 3, a retainer ring 8, a rubber barrel 9, a diversion sleeve 10, a connection sleeve 12, a rubber ring 13 and a lower joint 14. The oil well separate-layer proration regulator is characterized in that a plunger sleeve 6 is arranged between the upper joint 1 and the retainer ring 8, and a regulation sleeve 4, a spring 5 and a plunger 7 are arranged between the plunger sleeve 6 and the outer pipe 3. The utility model has reasonable structural design, convenience in processing and reliable operation, when the regulator is used for assembling a separate-layer flow string with anchors, a common deep-well pump can be used for pumping oil from different layers, and thus the utility model simplifies process structure and is convenient for production management.