The utility model provides a double-layered sealing cap for an endoscope biopsy hole, which belongs to the technical field of medical instruments and comprises a sealing cap, a connecting tongue connected onto the sealing cap and a tongue end cover, and the sealing cap adopts a tubular structure and a double-layered sealing diagram is arranged therein. The double-layered sealing diagram can avoid air leakage of the sealing cap, prevent tissue fluid from spilling over and spurting, so that the sealing effect of the sealing cap is better, the liquid or tissue in the internal environment can be better withheld, and favorable sealing with the biopsy instruments can be realized. Additionally, the double-layered sealing cap is convenient, safe and reliable to use, and is good for improving the examination effect and accurate operation.