The utility model discloses a water-maze video imaging system which mainly comprises a maze barrel and a platform, wherein the maze barrel is internally provided with a determinant quadrant azimuth and the platform is fixed in one azimuth; the maze barrel is internally provided with water used for test and the water level is higher than the platform; the side wall of the maze barrel of each quadrant is provided with two or more light-transmission chambers; the light-transmission chambers arranged on the same quadrant are arranged vertically and have different shapes; and the light-transmission chambers arranged on different quadrants and on the same horizontal surface also have different shapes. In the utility model, the memory reference objects formed by the light-transmission chambers are directly fixed on the side wall of the maze barrel so as to form a standard visible reference system, thus leading the tested animal not to be disturbed by the environment during the swimming process and being more beneficial to forming stable space memory.