The utility model relates to a double rubber-barrel isolation packer which comprises an upper connector, a central shaft and an upper rubber barrel sleeved on the central shaft, wherein an upper thrust mechanism and an upper locking mechanism are respectively arranged at the upper part of the central shaft; and the double rubber-barrel isolation packer is characterized in that a lower connector is connected at the lower part of the central shaft, a lower rubber barrel, a lower thrust mechanism and a lower locking mechanism are respectively sleeved between the central shaft and the lower connector, wherein the lower thrust mechanism comprises a lower lock sleeve, a lower adjusting ring and a lower wedge-shaped ring which are respectively sleeved on the central shaft in sequence, and the lower locking mechanism comprises a central shaft and a lower lock sleeve and a locking ring which are respectively sleeved on the central shaft. The utility model has the advantages that the packer achieves the purpose that the double sets of the thrust mechanisms are pushed by the well completion fluid through a group of pressure transfer holes to cause the two groups of the rubber barrels to be respectively expanded at the upper part and the lower part of the packer to clamp the casing pipe, ensures the reliable setting and the smooth working of the well-completion operation and enhances the sealing performance and the reliability of the upper and the lower oil and gas reservoirs during the well-completion operation; and the packer is simple and convenient to operate and secure and reliable in sealing, increases the hanging force and ensures the operating efficiency of production.