The utility model relates to a wood soundproof mortise-and-tenon door frame, comprising a top board and a side board, wherein the top board and the side board are made of boards with the same step sections; the vertical surface of a step is provided with a soundproof groove which is internally provided with soundproof strips; the horizontal surface of the step is a door stop, and a door leaf is arranged on the step; and a mortise-and-tenon connecting mode is adopted between the top board and the side board. The wood soundproof mortise-and-tenon door frame is simple and fast in installation, does not need fixing pieces such as nails and the like, is firm and durable, has the functions of vibration resistance, soundproof performance and sealing performance and the like, and is attractive and elegant.