A 48V powered breaker simulation box with an anti-tripping function is especially used for testing of a distribution network protection device, and mainly consists of a tripping/closing loop, a time-delay anti-interference loop, an energy dissipating loop and an anti-tripping loop. The 48V breaker simulation box comprises: a lockup relay CGJ for performing simulation of tripping/closing motions, capacitors C1 and C2 for performing time-delay anti-interference processes, energy dissipating resistors R2, R3, R5, and R6 for increasing a starting power, and a relay TBJV2 for realizing the anti-tripping function. The 48V breaker simulation box can completely simulate process of the breaker motions, and has the advantages of time-delay anti-interference capacity, large starting power, anti-tripping function, etc., thereby effectively ensuring accuracy of experiment results and safety of testing equipment.