The utility model discloses a queen bee isolation board which adopts the scheme that frame lug thickening sheets (6) are additionally mounted on the frame lugs at the two ends of a grid outer frame upper beam (1); frame lug thickening sheets (6) are additionally mounted at the junctions of the grid outer frame upper beam (1) and side strips (3); frame lug thickening sheets (6) are additionally mounted at the junctions of a grid outer frame lower beam (2) and the side strips (3); and the frame lug thickening sheets are made of resin. The queen bee isolation board has the advantages that since the frame lug thickening sheets are additionally mounted, so that the queen bee isolation board is not easy to damage when being detached, the service life of the queen bee isolation board is prolonged, and the bee-keeping cost is reduced; and compared with the thickening sheets made of iron, the thickening sheets made of resin has the advantage that heavy metal can not be led into the honey, so that the honey is safe to eat.