The utility model discloses an anti-reflux blood return transfusion system, relating to a medical transfusion system. The anti-reflux blood return transfusion system comprises a transfusion bottle, a transfusion pipe and a puncture needle, wherein the lower mouth of the transfusion bottle is connected with the transfusion pipe, a drip cup is serially connected in the transfusion pipe, a transfusion governor is arranged on the transfusion pipe, the lower end of the transfusion pipe is connected with the puncture needle, an anti-return valve is arranged at the root of the puncture needle and connected with the transfusion pipe, a valve base is arranged in a tubular column of the anti-return valve, and a floater is arranged at the lower mouth of a valve base. The anti-reflux blood return transfusion system provided by the utility model has the advantages of reasonable structure design, no influence on puncture transfusion and capability of maximally preventing blood reflowing, realization of finally eliminating a small sludged blood blocked pin hole and thrombus.