The utility model relates to a reverse centrifugal jelly taking device, which comprises a barrel body, a crank handle, a centrifugal turntable, a clamp groove, a queen table seat and queen tables and is characterized in that the queen tables are uniformly distributed on the queen table seat, the queen tables and the queen table seat are connected into a whole, a through hole is arranged on the queen table seat and is communicated with the queen tables, the diameter of the through hole is smaller than the diameter of the queen tables, a support table corresponding to the through hole of the queen table seat is arranged on a bee support device and can be inserted into the through hole of the queen table seat, during the jelly taking, the queen table seat is inserted into the clamp groove arranged on the centrifugal turntable, the queen tables face the inside, and the through hole faces the outside. The reverse centrifugal jelly taking device has the advantages that young queen bees do not need to be clamped out from the queen tables, the royal jelly in the queen tables is firstly separated out through reverse centrifugation, and then, the young queen bees are collected, so the young queen bees are not injured, and in addition, the quality of the royal jelly is ensured.