The utility model provides an opposite-clamping type simulated chewing machine. The opposite-clamping type simulated chewing machine is characterized by comprising a testing chamber which consists of a cylindrical water bath circulation box and an annular water bath circulation box surrounding the cylindrical water bath circulation box, a small bowl for holding both artificial saliva and a test sample is put on the cylindrical water bath circulation box, a pneumatic clamp connected with the rotating shaft of a motor extends into the bowl cavity in a suspended manner, a liquid inlet pipe and a liquid outlet pipe are arranged on two sides of the bowl cavity, and the bottom of the cylindrical water bath circulation box is connected with a cylinder and can move up and down under the action of the cylinder. The machine can simulate the constant-temperature oral cavity environment of a human being, simulate the chewing behavior of a human being, achieve the effect of completely stirring and chewing a tobacco chewing gum product with the artificial saliva to promote full release of chemical components in the tobacco chewing gum product, collect the chewing liquid for analysis and detection in real time, and automatically supplement new artificial saliva. The machine can be widely applied to the researches on chewing release behavior of different chewing foods and pharmaceuticals.