The utility model relates to a heat-resisting coliform test bottle, and mainly solves the problems that the conventional heat-resisting coliform test instrument is complex in structure and operation procedure and is high in cost. The heat-resisting coliform test bottle is characterized in that a cross plate (5) with a water sample through hole (8) and a guide tube through hole is fixed in a transparent glass bottle (1); an inverted test tube (9) passes through the guide tube through hole in the center of the cross plate (5) and is fixed; a counterweight block (7) capable of guaranteeing vertical stability of the test tube is fixed at the open end of the test tube (9); a sealing rubber plug (3) is plugged into the opening of the transparent glass bottle (1); an aluminum lid (4) which is used for sealing the opening is in threaded connection with the outside of the opening; and heat-resisting coliform test liquid (2) is accommodated in the bottle. The heat-resisting coliform test bottle has the characteristics of low cost, high efficiency, capacity of testing coliforms simply, quickly and accurately, long preservation time and convenience for use and carrying.