The utility model discloses a rapid cooling device for a culture medium preparation device, belonging to the field of disinfecting and sterilizing equipment, in particular relating to a cooling device for a culture medium preparation device. The rapid cooling device for the culture medium preparation device comprises a pipeline pump (1), a plate-type heat exchanger (6) and an electromagnetic valve (4) which are connected in sequence, wherein one end of the electromagnetic valve (4) is connected with a cold water inlet (7) of the plate-type heat exchanger (6), and the other end of the electromagnetic valve (4) is connected with a circulating water storage device to form a circulating water inlet pipeline; a water outlet (8) of the plate-type heat exchanger (6) is connected with the circulating water storage device through the other pipeline to form a circulating water outlet pipeline; a circulating water inlet (5) is connected with a water discharging hole of a reaction tank of the culture medium preparation device through the pipeline pump (1); and a water outlet (9) is connected with a water inlet of an evaporator through a pipeline. The rapid cooling device has the advantages that rapid cooling can be realized, cooling water can be effectively and circularly utilized and the like. Not only is the production quality of a culture medium ensured, but also a great deal of cooling time is saved.