The utility model discloses a cold and hot pulse fatigue tester of a barrier material. The cold and hot pulse fatigue tester comprises the following structures: an electric control system (1), a lifting rotary device (2), a sample frame and a test groove, wherein the electric control system (1) and the lifting rotary device (2) are integrally connected; the lifting rotary device (2) of which a rotating axis is distributed in a vertical direction is fixedly provided with a sample frame, and the sample frame is fixedly provided with a sample groove (5) used for placing a sample (6); and a test groove used for placing the sample (6) is arranged below a rotating path of the sample frame on the outer side of the rotating axis of the lifting rotary device (2). The cold and hot pulse fatigue tester, provided by the utility model, has the advantages of high automation degree, good temperature stability and excellent technical effect.