The utility model discloses a cell biology reactor sampler. The cell biology reactor sampler comprises a sampling tube, a sample introduction tube, a sample introduction valve, air filters and an air pump. The sampling tube comprises a tube body and a top cap. A liquid inlet and an air port are formed in the top cap. Two ends of the sample introduction tube are respectively a sample introduction end and a sample discharge end. A sample introduction end port is connected with the liquid inlet of the top cap. The sample discharge end is used for being connected with a liquid outlet of a cell biology reactor. The sample introduction valve is arranged on the sample introduction tube. The sample introduction valve is opened or closed so that a pipeline between the sampling tube and the cell biology reactor is communicated or disconnected with a communicating pipeline. The air pump is connected with the air port of the top cap through one of the air filters. The sampler can carry out sampling in a one-hundred-grade aseptic environment, and besides, nutrient solution residue is avoided so that the sampler can be prevented form being contaminated by infectious microbes in sampling for a next time.