The utility model discloses an obese gene detection chip which comprises a substrate provided with a detection surface, and a detection layer overlapped on the detection surface of the substrate, wherein the detection layer comprises a plurality of SNP (Single Nucleotide Polymorphism) identification combinations; each SNP identification combination is provided with at least two sensing regions, one sensing region is provided with a first nucleotide probe, the other sensing region is provided with a second nucleotide probe, and sequences of the first nucleotide probe and the second nucleotide probe of each SNP identification combination comprise SNP sites of an obese gene, and are different in one nucleotide. The obese gene detection chip is capable of rapidly and accurately detecting the obese gene and evaluating the risk of anti-obesity agents, and is applied to a large quantity of automatic detection or medical screening.