The utility model relates to a temperature tracing experiment device of the interaction between river water and underground water. The temperature tracing experiment device comprises a sand groove, a water flow controlling mechanism, a water level monitoring mechanism, and a boundary temperature controlling mechanism. The front panel of the cuboid sand groove is equipped with pressure measuring holes, a river water incoming and outgoing hole, boundary water level monitoring holes of underground water and river water. The back panel of the sand groove is equipped with a solute tracer hole. Underground water incoming and outgoing holes and correction dial gauges are disposed on the left panel and the right panel of the sand groove. An organic glass filtering board is mounted between the left panel and the right panel. A simulated riverbed is mounted on the top of the sand groove. The water flow controlling mechanism provides water for an underground water and river water stable supplying tank with a miniature water pump. The water level monitoring mechanism comprises the correction dial gauges and a pressure measuring plate. The boundary temperature controlling mechanism is used for controlling the boundary temperature at the bottom of the sand groove in order to make the vertical temperature gradient in the sand groove approach natural geothermal gradient. The device can be used in experiment teaching of the aspect of temperature tracing of underground water in universities, and indoor temperature tracing research of the interaction between the river water and the underground water so as to provide guidance for outdoor researches.