The utility model is suitable for the field of sterile sampling devices and provides a sterile sampling bottle which comprises a container bottle and a bottle cap, and also comprises a sampling pipe and a breathing pipe one ends of which penetrate through the bottle cap and are inserted into the container bottle respectively, the sampling pipe and the breathing pipe are in sealing connection to the bottle cap, a microporous filter membrane filter is connected to the other end of the breathing pipe, the other end of the sampling pipe is connected with a sampling valve, the other end of the sampling valve is connected with a t-branch pipe, one branch of the t-branch pipe is connected with a feeding valve, another branch of the t-branch pipe is connected with a drain valve, and the other end of the feeding valve is connected with a sampling chuck. The sampling pipe and the breathing pipe are arranged and the microporous filter membrane filter is connected to the breathing pipe, and thus infectious microbes are prevented from entering the container bottle through the breathing pipe. The t-branch pipe is connected to the sampling pipe, the sampling valve, the feeding valve and the drain valve are respectively connected to three branches of the t-branch pipe, the other end of the feeding valve is connected with the sampling chuck, and thus the container bottle, the t-branch pipe and the sampling chuck are firstly sterilized and disinfected during the sampling process, thereby ensuring that infectious microbes cannot be introduced in the obtained sample.