The utility model discloses a device for destacking bottles in a layered mode. The device for destacking the bottles in the layered mode comprises a machine frame, a bottle platform delivering mechanism, a bottle stack delivering mechanism, a lifting mechanism and a gripping fixture destacking mechanism. The gripping fixture destacking mechanism is installed on the machine frame in a sliding mode. The lifting mechanism drives the gripping fixture destacking mechanism to move vertically. The bottle stack delivering mechanism delivers a bottle stack to the portion below the gripping fixture destacking mechanism. The bottle platform delivering mechanism is arranged below the machine frame and outputs the layered bottles. Compared with the prior art, the device for destacking the bottles in the layered mode can relieve the intensity of the operation labor carried out by personnel, improves the automation degree and production efficiency, and stabilizes the quality safety of products due to the fact that facility automation replaces hand-operated bottle feed.