The utility model discloses a contamination cylinder for a sulfur hexafluoride biological test. The contamination cylinder comprises a cylinder body and a cylinder cover sealing the cylinder body, wherein an isolating layer is arranged in the cylinder body; a supporting leg for supporting the isolating layer is fixed below the isolating layer; at least one groove is formed in the isolating layer; through a plurality of seams or holes formed in the groove, the groove is communicated with the lower space of the cylinder body below the isolating layer; the cylinder cover is provided with a glass cover, an air inlet pipe and an air outlet pipe; both the air inlet pipe and the air outlet pipe are made of glass; the glass cover is in sealing connection to the cylinder cover, and the glass cover is communicated with the cylinder body; the air inlet pipe sequentially runs through the glass cover and the isolating layer until stretching to the lower side of the cylinder body; the air outlet pipe is communicated with the glass cover. The contamination cylinder disclosed by the utility model, through the structure, is easy to clean and disinfect, and the contamination cylinder isolates drinking water and feed from a moving region of mice, so as to guarantee accuracy of a test conclusion.