The utility model discloses an isolation membrane-type isolation bag. The isolation membrane-type isolation bag comprises an isolation bag body, wherein an isolation membrane is arranged in the isolation bag body; the isolation bag body is divided into two accommodating chambers by the isolation membrane; one accommodating chamber is a measured medium accommodating chamber; the other accommodating chamber is an isolation liquid accommodating chamber; a measured medium leading-in opening is formed in the measured medium accommodating chamber; an isolation liquid filling opening is formed in the isolation liquid accommodating chamber; the isolation liquid filling opening also serves as a measurement instrument access port. A measured medium accommodating chamber exhaust hole is also formed in the measured medium accommodating chamber; an isolation liquid accommodating chamber exhaust hole is also formed in the isolation liquid accommodating chamber; the isolation membrane is a spherical isolation membrane; a spherical isolation membrane protective mesh is arranged in a position, which is 5 mm to each of the two sides of the spherical isolation membrane; the two protective meshes are respectively welded on the inner walls of the two accommodating chambers. By adopting the isolation membrane-type isolation bag, the stability of a pressure-leading system can be greatly improved, and meanwhile, the problem that the pressure-leading system is frozen caused by the fact that the anti-freezing performance of the isolation liquid is reduced because the isolation liquid is diluted in winter is solved.