The utility model discloses a magnetic separation frame with demagnetizing capability. The magnetic separation frame comprises two independently combined parts, namely a centrifuge tube fixing frame and a magnetic insertion strip, wherein a plurality of centrifuge tubes of different specifications can be placed on the centrifuge tube fixing frame, and a plurality of permanent magnets are fixedly arranged on the magnetic insertion strip. According to the magnetic separation frame, a magnetic insertion strip penetrating hole is formed in a side plate with centrifuge tube holes, so that functions for magnetizing and demagnetizing the centrifuge tubes placed on the centrifuge tube fixing frame can be conveniently realized by virtue of insertion and pull-out of the magnetic insertion strip; the magnetic separation frame is attractive in appearance and small and exquisite in volume, is light, convenient and rapid to operate, can be used for easily demagnetizing a to-be-processed sample and is applicable to the operation of the centrifuge tubes of different specifications.