The utility model discloses a collection contact type fluorescence detection pool for capillary electrophoresis. The collection contact type fluorescence detection pool comprises a detection pool body assembly, a locating assembly and a fixing assembly. The collection contact type fluorescence detection pool is of an orthogonal light path structure to ensure that excitation and collection optical fibers and liquid light paths are perpendicular, conjugated and intersected pairwise. A conical reducing tee made of a quartz glass material is connected with an inlet capillary tube, an outlet capillary tube and a collecting optical fiber, and thus the physical contact of a liquid flow path and the collecting optical fiber is realized; meanwhile, a detection region is always made of a quartz material, the continuous operation of an electroosmotic flow can be ensured, and the collection contact type fluorescence detection pool is suitable for capillary electrophoresis-fluorescence detection; in addition, a locating element, the fixing assembly and the like matched with the collection contact type fluorescence detection pool are designed, and thus the self alignment of the excitation optical fibers and the detection region is realized, and the detection pool is relatively conveniently installed and used.