The utility model relates to the field of medical instruments, and particularly relates to a culture dish sterilization box. The culture dish sterilization box comprises a box body and a box cover matched with the box body, wherein a plurality of partition walls are arranged inside the box body; the left side and the right side of the box body are provided with ventilation devices; the heights of the partition walls are smaller than that of the box body; and the ventilation devices are positioned at the positions, which are higher than the partition walls and lower than the box body, on the box body. According to the culture dish sterilization box, the partition walls are arranged inside the box body, and the left side and the right side of the box body are provided with the ventilation devices, so that the target of conveniently sterilizing the culture dish is achieved. The culture dish sterilization box is simple in structure, convenient to use and high in practicability.