The utility model provides an anti-skidding sealing device. The anti-skidding sealing device comprises a detachable rubber sealing plug, a fixing bolt, a nut, a fastening clamp or a fastening frame and a stainless steel probe, wherein the detachable rubber sealing ring is conical; the fastening clamp is a metal spring clamp; metal support frames of which the included angle is 90 degrees are arranged on two swings on the side face of the fastening clamp; a metal sheet with an included angle of 90 degrees is taken as the fastening frame; the metal sheet is provided with a hole which is matched with the fixing bolt; the detachable sealing rubber plug is fixedly integrated with the fastening frame through the bolt and the nut; the stainless probe sleeves the inside of the detachable rubber sealing plug for fixing. The anti-skidding sealing device has the advantages of simple structure, convenience and safety in use, difficulty in moving the probe and high fixing performance.