The utility model relates to a transformer insulation isolation safety barrier. An object of the utility model is to provide an isolation barrier with a simple structure, high safety and a wide application range. The transformer insulation isolation safety barrier comprises angle steel clamps, two connection support rods, a cross beam, two outer stand columns and a plurality of barrier connection rods. A strip-shaped groove is formed at the lower side of the cross beam which is horizontally arranged. The two outer stand columns are mounted vertically in the groove. The plurality of barrier connection rods are hinged between the two outer stand columns. The top ends of two outermost barrier connection rods are hinged to the top ends of the two outer stand columns. The two outer stand columns and the plurality of barrier connection rods are movable in the groove in the left-right direction. The two connection support rods are arranged at the rear ends of the two outer stand columns, respectively. One end of each connection support rod is fixedly connected with one outer stand column, and one angle steel clamp is mounted at the other end of the connection support rod. Each angle steel clamp is provided with a bolt. The connection support rods, the cross beam, the outer stand columns and the barrier connection rods are all made of insulating material.