The utility model discloses a liquid chromatographic separation column and belongs to the technical field of liquid chromatogram. The utility model adopts the technical scheme as follows: the liquid chromatographic separation column comprises a separation column body, an upper chromatographic column head and a lower chromatographic column head, and is characterized in that a rough bulge is arranged on the inner side of the separation column body; a stationary-phase adsorbent is arranged on the inner side of the rough bulge; a column head fastening outer thread is formed on the upper end of the outer side of the separation column body; a column tail fastening outer thread is formed on the lower end of the outer side of the separation column body; an upper column head fixer is arranged on the lower side of the upper chromatographic column head; a fixer inner thread is formed in the inner side of the upper column head fixer. The liquid chromatographic separation column is simple in structure, can be used once or repeatedly used for multiple times, cannot cause leakage of a flowing phase, and has the advantages of simplicity and convenience for operation, low use cost, capability of greatly improving the production efficiency and the like.