The utility model relates to a rat fixing device for acupuncture and moxibustion. The rat fixing device comprises two base plates, a bottom groove with four supports and two transverse strips; the two transverse strips are fixed to the supports of the bottom groove respectively, a plurality of buckles are arranged on each transverse strip at intervals, and the two base plates are fixed between the two transverse strips through the buckles so that a rat can be horizontally lied on the two base plates. A fixing strip is arranged at the positions, close to the middle, of the base plates so as to be used for binding the rat; a black hood is arranged at the transverse strips between the two base plates so as to be used for covering the head of the rat when the rat is fixed. The rat fixing device for acupuncture and moxibustion is simple in structure, low in manufacturing cost, good in adjusting performance, convenient to disassemble and assemble and convenient to wash; the rat fixing device can provide comfortable fixing conditions for the rat, the rat rarely has irritation reactions, smooth implementation of the treatment process can be well guaranteed, the fundamental research reliability of the acupunctured rat is higher, and the rat fixing device is worthy of application and popularization in an experiment.