The utility model relates to the technical field of auxiliary tools of coiled tubing operation, in particular to a coiled tubing injection head tubing-penetrating calibrator, which comprises a plate seat, a nut seat, a screw rod, a mounting seat, a press wheel, a left pull grab and a right pull grab, wherein a mounting through hole is formed in the middle part of the plate seat, the nut seat is fixedly mounted in the mounting through hole, a screw rod is arranged in the nut seat and can move up and down along the nut seat, the lower end of the screw rod is positioned under the plate seat, and the mounting seat is mounted at the bottom part of the screw rod and can rotate around the axis of the screw rod. The coiled tubing injection head tubing-penetrating calibrator has the advantages that the structure is reasonable and compact; by rotating the screw rod, the distance between the press wheel and a limiting grab block can be adjusted, so the calibrator and a coiled tubing are limited on a gooseneck, the penetrating end of the coiled tubing is aligned with a tubing inlet of an injection head by adjusting the distance between the injection head and the gooseneck, and the penetrating of the coiled tubing is easily realized; the operation can be independently completed by one person, the limitation of space on installation is reduced, the easiness and convenience are realized, the time and labor are saved, the rapidity is realized, and the efficiency is high.